Plasma Seed Technology is a novel, eco-friendly pre-sowing method that employs non-thermal plasma to improve seed performance without relying on chemicals. This research intended to assess the effect of plasma exposure time and seed positioning (with or without rotation) on germination and initial seedling strength in tomato (Solanum lycopersicum), brinjal (Solanum melongena), and chilli (Capsicum annuum). Seeds underwent cold plasma treatment for different exposure durations (4 to 32 seconds), with and without rotation, alongside an untreated control. Fourteen plasma treatments were assessed, and the impacts on germination rate, average germination duration, seedling height, and vigour index were noted under regulated conditions. Of all treatments, 16 seconds of plasma exposure without flipping consistently surpassed others in all three crops. This therapy greatly enhanced germination rates and seedling strength in comparison to the control and other plasma exposure times. The enhancement is linked to the improved interaction of Reactive Oxygen and Nitrogen Species (RONS) generated by plasma with the seed surface, which increased seed coat permeability, activated enzymes, and achieved microbial decontamination, while avoiding oxidative harm that could arise from prolonged exposures. The results indicate that a 16-second plasma treatment without flipping is an effective and optimal method for improving seed quality and initial seedling development in solanaceous vegetables. This method has significant potential to be incorporated into commercial seed treatment programs as an environmentally friendly substitute for chemical priming techniques.