The present investigation entitled “Effect of sulphur levels on growth, yield and quality of different varieties of garlic (Allium sativum L.)” was carried out at the Research Farm, School of Agricultural Sciences, Jaipur National University, Jaipur. The experiment aimed to study the influence of different sulphur levels and varietal responses on growth, yield, and quality parameters of garlic. The treatments consisted of four sulphur levels (0, 20, 30, and 40 kg S/ha) and four varieties (Riyawan, Ooty, Mahadev, and G-2) arranged in a factorial randomized block design. The results revealed that the application of sulphur significantly enhanced plant growth traits such as leaf length and number of leaves, along with yield attributes including bulb diameter, number of cloves, bulb weight, and total yield. The maximum values for these parameters were recorded with 40 kg S/ha, followed by 30 kg S/ha. Among the varieties, G-2 performed best in terms of yield and quality parameters such as total soluble solids, dry matter content, and sulphur concentration in bulbs. Economic analysis also showed that the highest net return and benefit-cost ratio were obtained with 40 kg S/ha in variety G-2. Thus, it can be concluded that the application of sulphur at 40 kg/ha is optimum for maximizing growth, yield, and quality of garlic under Jaipur agro-climatic conditions.