Under varying climatic conditions, plant parasitic nematodes (PPNs) cause substantial economic damage to a wide variety of agricultural and horticultural crops. A survey of polyhouses in different districts of Haryana was conducted during 2020-25 to determine the incidence of important PPNs on vegetable crops mainly, cucumber, tomato, chili and bell peppers grown in protected conditions. In this respect, a total of 250 roots and rhizosphere soil samples were collected from the surveyed plants such as cucumber, tomato, chili and bell peppers from different districts (Jind, Kaithal, Bhiwani, Hisar, Fatehabad and Palwal). Soil and root samples were collected from each polyhouse and analyzed mainly for the presence of PPNs. Out of these, 178 samples were found infested withroot-knot nematodes (RKNs) with 71.2% frequency of occurrence. Frequency of occurrence of each species was highly variable from field to field. During the survey, different PPNs genera viz., Meloidogyne, Pratylenchus, Rotylenchulus, Hoplolaimus, Helicotylenchus, Tylenchorhynchus were detected from soil samples. Based on incidence, population density and associated damage on affected crops, M. incognita was considered to be the most important parasites of the crops under local condition. The average population density range of RKN was recorded to be 70-670 J2s/200cc soil. The frequency of occurrence of M. incognita was 82.5% in Fatehabad, 73.3% in Jind, 70.0% in Hisar, 68.0% in Kaithal, 60.0% in Bhiwani and 50.0% in Palwal district, respectively.